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To investigate the effect of Aloe polysaccharide on proliferation and hyaluronic acid and hydroxyproline secretion of human fibroblasts in vitro.
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As a rising medical science,evidence-based medicine should be extensively used.Applying the principle of evidence-based medicine to the clinical teaching of burns has positive significance for improving teaching quality,which is helpful to the cultivation of students'clinical thinking abilities.
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Aim To investigate the effects of aloe coarse polysaccharid on proliferation and the intracellular calcium level and the leakage rate of lactate dehydrogen-ase(LDH) of the epidermal cellsin vitro.Methods The epidermal cells were treated with aloe coarse po-lysaccharid of75,150,300,600 and 1 200 mg.L-1respectively.The proliferation of epidermal cells wasobserved interms of the proliferation curve and the pop-ulation doubling/day(PD/d).The concentration ofintracellular calcium and the leakage rate of LDHwerealso measured.Results In aloe coarse polysaccharidgroup,a dose-dependent and statistically significant(P
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Aim To investigate the influence of oxygen-free radical to erythrocyte immune adherent function after severe burns. Methods The erythrocyte's immune adherent function (RC3bRR and RICR) and the levels of SOD, Lpo-MDA, CAT, GSH-px and GSH were detected on 1, 3, 5, 7, 14, 21, 28 and 35 days after severe burns, and then their correlations were analyzed. Results The rosette forming rate of erythrocyte C3b receptor (RC3bRR) in post-burn group was obviously lower than that in normal control group (P＜ 0.05～ 0.001), and erythrocyte immune complex rosette forming rate (RICR) of the burned patients was obviously higher than that of the healthy group(P＜ 0.001), but they gradually returned to normal four weeks after burn. Compared with healthy control group, the levels of SOD, CAT, GSH-px and GSH markedly declined(P＜ 0.05～ 0.001), while Lpo-MDA level notably increased(P＜ 0.05～ 0.001), but their recovery needed at least three weeks. RC3bRR was positively correlated with the levels of SOD, CAT, GSH-px and GSH, and was negatively correlated with Lpo-MDA. Correlation of RICR was contrary to that of RC3bRR. Conclusion The degree of the free radical injury may directly influence the erythrocyte membrane function, especially the erythrocyte immune adherent. It is necessary to reducing the production of free radicals and enhacing patient's erythrocyte immune function during burn treatment.
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Objective To investigate the change of insulin-like growth factor Ⅱ(IGF-Ⅱ) and its relation with infection in severely burned patients.Methods RIA method was used to determine the serum IGF-Ⅱ level in patients with severe burn.Results Compared with control group, the level of IGF-Ⅱ was increased significantly at 5 postburn days , and persistent 35 days (P0 05).Conclusion IGF-Ⅱ is involved in the pathogenesis of burn , and closely correlated with the infection after burn.
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Objective:To investigate the change of the serum IL-18, sFas and sFasL levels in patients with severe burn and its correlation with the morbid condition and outcome Methods:Thirty patients, including three fatal cases, had burn with a total burn surface area (TBSA) of 30% or more 45 healthy volunteers were as control group The serum IL-18, sFas and sFasL levels were measured by enzyme-linked immunosorbent assay (ELISA) at various time points ( 1, 3, 5, 7, 14, 21, 28 and 35 days) burn later Results:Compared with control group, the levels of IL-18, sFas and sFasL of patients increased significantly after burn (P